Secondary Antibodies
Secondary antibodies are an essential tool for indirect detection in immunochemical methods (WB, ELISA, IHC, IF). These antibodies are directed against a primary antibody (e.g., Anti-Mouse IgG, Anti-Rabbit IgG) and are conjugated (labeled) with an enzyme (HRP, AP) or fluorophore for signal visualization. Do you need a highly sensitive HRP-conjugated secondary antibody for chemiluminescent detection on Western Blot, or a fluorescently labeled antibody for microscopy? Find the right conjugated secondary antibody (e.g., from Elabscience) that recognizes your primary antibody here. All products can be found here.
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Secondary antibodies serve as a "bridge" between the primary antibody and the detection system, providing vital signal amplification through indirect detection.
Selection Criteria
- Host Species: Must match the source of the primary antibody (e.g., Anti-Mouse for a mouse primary antibody).
- Conjugates: HRP for Western Blotting, AP for colorimetry, or fluorophores for immunofluorescence (IF) from labeling kits.
- Specificity: Affinity-purified versions ensure a clean signal with low background noise.
